tRNA-like structures in 10Sa RNAs ofMycoplasma capricolumandBacillus subtilis

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A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli.

We have determined that 10Sa RNA (one of the small stable RNAs found in Escherichia coli) has an interesting structural feature: the 5' end and the 3' end of 10Sa RNA can be arranged in a structure that is equivalent to a half-molecule (acceptor stem and TFC stem-loop) of alanine tRNA of E. coli. Primer-extension analysis of 10Sa RNA extracted from a bacterial mutant with temperature-sensitive ...

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The surprise was great in the early 1970s when it was shown that valine could be covalently attached to the 3' terminus of the genomic RNA from turnip yellow mosaic virus (TYMV) by valyl-tRNA synthetase (1) and, soon after, that tyrosyland histidyl-tRNA synthetases can aminoacylate the RNAs from brome mosaic virus (BMV) and tobacco mosaic virus (2, 3). The surprise was even greater when it was ...

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Identification of regulatory RNAs in Bacillus subtilis

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Computational Approaches to tRNA-Derived Small RNAs

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The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subunits and form virions was assayed in vitro. In contrast to the well established...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1994

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/22.16.3392